anti igfbp5  (R&D Systems)

Journal: Cell reports

Article Title: Tissue differences in the exosomal/small extracellular vesicle proteome and their potential as indicators of altered tissue metabolism

doi: 10.1016/j.celrep.2021.110277

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The following antibodies were used: ADIPOQ (AB3269P, Millipore-Sigma), CD36 (ab133625, Abcam), HSP90alpha (PA3–013, Thermofisher), POSTN (ab14041, Abcam), PGAM1/4 (sc-376638, Santa Cruz); THBS1 (14778, Cell Signaling), ITGB1 (4706, Cell Signaling), EGFR (2232, Cell Signaling), SPARC (8725, Cell Signaling), IGFBP5 (AF578, R&D systems), CD9 (ab92726, Abcam), CD63 (ab68418, Abcam), CANX (ab22595, Abcam) and FASN (ab22759, Abcam).

Techniques: Western Blot, Recombinant, Enzyme-linked Immunosorbent Assay, Mass Spectrometry, Software

Journal: Circulation research

Article Title: Transcriptomic Profiling of the Developing Cardiac Conduction System at Single-Cell Resolution

doi: 10.1161/CIRCRESAHA.118.314578

Figure Lengend Snippet: (A) Cluster cell numbers and t-SNE plot for Zone I cells. (B) Heatmap of differentially expressed genes for each cluster of cells. (C) Cardiomyocyte (Actn2+) and Nodal (Hcn4+, Isl1+, Shox2+, Tbx3+) signatures visualized by ViolinPlots (top) and FeaturePlots (bottom). (D) Table highlighting differentially expressed genes in Cluster 9. Avg log FC, average log fold change. (E) Gene ontology (GO) functional cluster analysis of all enriched genes for Cluster 9. (F) Immunofluorescence staining of postnatal day 4 (P4) (n=8) wild-type murine cardiac tissue sections showing a SAN with colocalization of Hcn4 (green) and Igfbp5 (red) protein. Nuclei stained with DAPI (blue). RA, right atrial myocardium.

Article Snippet: Primary antibodies used included: Anti-mouse Igfbp5 Goat Polyclonal Antibody (R&D systems/Fisher Scientific: AF578) at 1:100 dilution; Anti-mouse Connexin 40 Rabbit Polyclonal antibody (Alpha Diagnostics, Cx40-A) at 1:100 dilution; Anti-mouse Hcn4 Rat Monoclonal [SHG 1E5] antibody (Abcam, ab32675) at 1:75 dilution.

Techniques: Functional Assay, Immunofluorescence, Staining

Journal: Circulation research

Article Title: Transcriptomic Profiling of the Developing Cardiac Conduction System at Single-Cell Resolution

doi: 10.1161/CIRCRESAHA.118.314578

Figure Lengend Snippet: (A) Schematic representation of workflow for iDISCO+ clearing of intact embryonic and postnatal mouse hearts and visualization using light sheet microscopy. At least 10 biological replicates were used for each immunolabelling. (B) A wild-type whole heart from an E16.5 mouse embryo is shown at four angles of view (0, 90, 180 and 270 degrees) at 0.63x magnification. Top and bottom rows are the same optically cleared heart using iDISCO+ where, in the top row, background fluorescence has been saturated to provide a representation of the opacified heart. Bottom row demonstrates the same tissue-cleared heart, showing Igfbp5 immunostaining which marks the cardiac conduction system in its entirety. (C) iDISCO+ cleared wild-type, intact SAN (6.3x magnification) from E16.5 murine heart co-immunolabeled for Hcn4 (red) and Rgs6 (green) protein. SAN shown at three angles of view (0, 90, ~225 degrees). (D) Merge image (Hcn4 in red, Rgs6 in green) with two major transitional sinoatrial conduction pathways (SACPs) outlined (Hcn4neg/Rgs6+). Purple SACP: from SAN body directed rightward; Black SACP: from the SAN head directed inferiorly and leftward. A third Hcn4+ SACP is also marked by Rgs6 and emerges inferiorly from the tail of the SAN to give rise to the internodal tracks (blue line) not seen here but visible in panel 8B. Ant, Anterior. AVN, atrioventricular node. His, His bundle. INT, internodal tracks. LA/RA, left or right atrium. LAVRB, left AV ring bundle. LBB/RBB, left of right bundle branch. LV/RV, left or right ventricle. PF, Purkinje fiber. Post, Posterior. RAVRB, right AV ring bundle. SAN, sinoatrial node.

Article Snippet: Primary antibodies used included: Anti-mouse Igfbp5 Goat Polyclonal Antibody (R&D systems/Fisher Scientific: AF578) at 1:100 dilution; Anti-mouse Connexin 40 Rabbit Polyclonal antibody (Alpha Diagnostics, Cx40-A) at 1:100 dilution; Anti-mouse Hcn4 Rat Monoclonal [SHG 1E5] antibody (Abcam, ab32675) at 1:75 dilution.

Techniques: Microscopy, Fluorescence, Immunostaining, Immunolabeling